The BIOCYCLING project aims to develop a biofilm-based bioprocess to convert polyethylene terephthalate (PET) waste into valuable chemical compounds. Focusing on microbial degradation, the project investigates biofilm formation on PET surfaces and how bacterial structure and physiology influence degradation efficiency.

A key aspect is the development of bacterial strains, particularly Comamonas thiooxidans, capable of robust PETase secretion and PET metabolism. Based on these advances, a continuous bioreactor system is being designed, where biofilms degrade PET and convert terephthalic acid into 3,4-dihydroxybenzoic acid (PCA). To enable accurate process control, in-line analytical methods such as FTIR and NMR are optimized for real-time detection of degradation intermediates and products. Through this interdisciplinary research, the project aims to establish an efficient and scalable system for sustainable PET upcycling. Its three main objectives are to understand biofilm adhesion, engineer PET-degrading bacteria, and optimize bioreactors for continuous conversion of plastic waste into valuable chemicals.