Filamentous cyanobacteria of the genus Nostoc have unique advantages as biofilm producers. They form robust high-density biofilms, fix nitrogen, and exhibit pronounced multicellularity and phenotypic plasticity. These properties can be harnessed for efficient spatial division of labor and sustainable chemical production. This project investigates how mono- and multi-species biofilms can be engineered to produce bulk and fine chemicals at high titers. A central paradigm is the use of spatial metabolomics, applying advanced mass spectrometry imaging to understand and control heterogeneity in biofilms. By correlating metabolic gradients with production hotspots, the project aims to decouple growth and product formation, thereby offering an avenue to increase efficiency. Outcomes will include a standardized pipeline for spatial metabolomics in biofilms and proof-of-concept production of compounds such as pyreudione A, cyanobacterin, and limonene. This will open new opportunities for sustainable, biofilm-based production of fine chemicals.